Mated: a putative peptide transporter of Schizophyllum commune expressed in dikaryons

Curr Genet. 1999 Sep;36(3):159-64. doi: 10.1007/s002940050486.

Abstract

A gene that exhibits sexually induced transcript accumulation, mtd1, was identified physically linked to a locus that is also under control of the mating-type genes. Genomic and cDNA sequence analyses were performed to determine a large open reading frame which starts with the amino-acid sequence M-A-T-E-D. The ORF showed similarities to a novel family of peptide transporters with at least 12 putative transmembrane structures. A gene disruption resulted in a growth-phenotype with slow aerial mycelium formation that could be complemented with peptone or uracil added to the growth medium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics*
  • Carrier Proteins / physiology*
  • Cloning, Molecular
  • Gene Deletion
  • Genes, Fungal / genetics*
  • Genes, Mating Type, Fungal*
  • Genetic Complementation Test
  • Membrane Transport Proteins*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Phenotype
  • Protozoan Proteins*
  • RNA / analysis
  • Schizophyllum / cytology*
  • Schizophyllum / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Carrier Proteins
  • Membrane Transport Proteins
  • Protozoan Proteins
  • mtd1 protein, Schizophyllum commune
  • RNA

Associated data

  • GENBANK/AF125094