Isolation of a novel SUMO protein from tomato that suppresses EIX-induced cell death

Plant J. 1999 Sep;19(5):533-41. doi: 10.1046/j.1365-313x.1999.00547.x.

Abstract

Challenging tomato or tobacco varieties with ethylene-inducing xylanase (EIX) from the fungus Trichoderma viride causes rapid induction of plant defence responses leading to programmed cell death. Using the yeast two-hybrid system, we isolated a novel protein, tomato small ubiquitin-related modifier protein (T-SUMO), which specifically interacts with EIX. T-SUMO, a cytoplasmic protein, is a member of the ubiquitin-like protein family. It shows homology to human protein sentrin/SUMO1, which suppresses tumour necrosis factor-induced cell death. Transgenic plants that express T-SUMO in the sense orientation suppress EIX induction of ethylene biosynthesis and cell death, while in the antisense orientation they enhance EIX-induced ethylene biosynthesis. These results indicate that T-SUMO is involved in mediating the signal generated by EIX that leads to induction of plant defence responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Death*
  • Chromosome Mapping
  • Ethylenes / biosynthesis*
  • Humans
  • Lycopersicon esculentum / chemistry
  • Lycopersicon esculentum / genetics*
  • Molecular Sequence Data
  • Plants, Genetically Modified
  • Ubiquitins / chemistry
  • Ubiquitins / isolation & purification*
  • Ubiquitins / metabolism*
  • Xylan Endo-1,3-beta-Xylosidase
  • Xylosidases / metabolism*

Substances

  • Ethylenes
  • SUMO protein, plant
  • Ubiquitins
  • ethylene
  • Xylosidases
  • Xylan Endo-1,3-beta-Xylosidase

Associated data

  • GENBANK/AJ012717