A generic protein purification method for protein complex characterization and proteome exploration

Nat Biotechnol. 1999 Oct;17(10):1030-2. doi: 10.1038/13732.


We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Affinity Labels
  • Amino Acid Sequence
  • Electrophoresis, Polyacrylamide Gel
  • Methods*
  • Molecular Sequence Data
  • Proteins / isolation & purification*
  • Proteome / chemistry*


  • Affinity Labels
  • Proteins
  • Proteome