Association of alterations in ParC and GyrA proteins with resistance of clinical isolates of Enterococcus faecium to nine different fluoroquinolones

S Brisse; A C Fluit; U Wagner; P Heisig; D Milatovic; J Verhoef; S Scheuring; K Köhrer; F J Schmitz

doi:10.1128/AAC.43.10.2513

Association of alterations in ParC and GyrA proteins with resistance of clinical isolates of Enterococcus faecium to nine different fluoroquinolones

Antimicrob Agents Chemother. 1999 Oct;43(10):2513-6. doi: 10.1128/AAC.43.10.2513.

Authors

S Brisse¹, A C Fluit, U Wagner, P Heisig, D Milatovic, J Verhoef, S Scheuring, K Köhrer, F J Schmitz

Affiliation

¹ Eijkman-Winkler Institute, Utrecht University, 3584 CX, Utrecht, The Netherlands. sbrisse@lab.azu.nl

Abstract

The parC and gyrA genes of 73 ciprofloxacin-resistant and 6 ciprofloxacin-susceptible Enterococcus faecium clinical isolates were partly sequenced. Alterations in ParC and GyrA, possibly in combination with other resistance mechanisms, severely restricted the in vitro activities of the nine quinolones tested. For all isolates, clinafloxacin and sitafloxacin showed the best activities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents / pharmacology*
DNA Gyrase
DNA Topoisomerase IV
DNA Topoisomerases, Type II / genetics*
Drug Resistance, Microbial / genetics
Enterococcus faecium / drug effects
Enterococcus faecium / genetics*
Fluoroquinolones
Humans
Microbial Sensitivity Tests
Mutation

Substances

Anti-Infective Agents
Fluoroquinolones
DNA Topoisomerase IV
DNA Gyrase
DNA Topoisomerases, Type II