Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis

P Scarpellini; S Braglia; P Carrera; M Cedri; P Cichero; A Colombo; R Crucianelli; A Gori; M Ferrari; A Lazzarin

doi:10.1128/AAC.43.10.2550

Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis

Antimicrob Agents Chemother. 1999 Oct;43(10):2550-4. doi: 10.1128/AAC.43.10.2550.

Authors

P Scarpellini¹, S Braglia, P Carrera, M Cedri, P Cichero, A Colombo, R Crucianelli, A Gori, M Ferrari, A Lazzarin

Affiliation

¹ Infectious Diseases Division, San Raffaele Scientific Institute, University of Milan, Milan, Italy. scarpellini.paolo@hsr.it

Abstract

We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Antibiotics, Antitubercular / pharmacology*
DNA, Bacterial / analysis
DNA-Directed RNA Polymerases
Drug Resistance, Microbial
Electrophoresis, Polyacrylamide Gel / methods*
Female
Humans
Male
Middle Aged
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Plant Proteins / genetics
Point Mutation
Polymerase Chain Reaction
Rifampin / pharmacology*

Substances

Antibiotics, Antitubercular
DNA, Bacterial
Plant Proteins
RpoB protein, Oryza sativa
DNA-Directed RNA Polymerases
Rifampin