Viability and DNA maintenance in nonculturable spiral Campylobacter jejuni cells after long-term exposure to low temperatures

B Lázaro; J Cárcamo; A Audícana; I Perales; A Fernández-Astorga

doi:10.1128/AEM.65.10.4677-4681.1999

Viability and DNA maintenance in nonculturable spiral Campylobacter jejuni cells after long-term exposure to low temperatures

Appl Environ Microbiol. 1999 Oct;65(10):4677-81. doi: 10.1128/AEM.65.10.4677-4681.1999.

Authors

B Lázaro¹, J Cárcamo, A Audícana, I Perales, A Fernández-Astorga

Affiliation

¹ Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV/EHU), 01080 Vitoria-Gasteiz, Spain.

Abstract

Survival of Campylobacter jejuni at 4 and 20 degrees C was investigated by using cellular integrity, respiratory activity, two-dimensional (2D) protein profile, and intact DNA content as indicators of potential viability of nonculturable cells. Intact DNA content after 116 days, along with cellular integrity and respiring cells, was detected for up to 7 months at 4 degrees C by pulsed-field gel electrophoresis. Most changes in 2D protein profiles involved up- or down-regulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / analysis
Campylobacter jejuni / genetics
Campylobacter jejuni / physiology*
DNA, Bacterial / analysis*
Electrophoresis, Gel, Pulsed-Field
Electrophoresis, Gel, Two-Dimensional
Temperature

Substances

Bacterial Proteins
DNA, Bacterial