Distribution of human CMV-specific memory T cells among the CD8pos. subsets defined by CD57, CD27, and CD45 isoforms

Eur J Immunol. 1999 Sep;29(9):2908-15. doi: 10.1002/(SICI)1521-4141(199909)29:09<2908::AID-IMMU2908>3.0.CO;2-8.

Abstract

Chronic antigenic stimulation has been associated with peripheral blood expansions of CD8pos. T cells characterized by CD57 expression, loss of CD27 expression, and reversal of the CD45RO(bright) /RA(dim) phenotype usually associated with immunological memory towards a CD45RO(dim) /RA(bright) phenotype. However, the relationship and functional significance of these subset(s) has remained controversial. Here, this issue was addressed using a novel flow cytometric technique that allows simultaneous detection of human cytomegalovirus (HCMV)-specific CD8pos. memory T cells by rapid (< 6 h) HCMV peptide-specific induction of cytokine synthesis, and their phenotypic characterization, including CD57, CD27 and CD45RA/RO. The vast majority of resting CD8(pos.) T cells capable of rapid induction of IFN-gamma and TNF-alpha synthesis in response to HCMV peptides were found in a subset characterized by intermediate to high expression of CD57, down-regulation/loss of CD27, and varying degrees of reversal of the classical "memory" CD45RO(bright) /RA(dim) phenotype. This subpopulation likely includes the fully differentiated memory cells responsible for the long-term immune defense against HCMV reactivation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / biosynthesis*
  • CD57 Antigens / biosynthesis
  • CD8-Positive T-Lymphocytes / chemistry
  • CD8-Positive T-Lymphocytes / metabolism*
  • Cytomegalovirus / immunology*
  • Flow Cytometry
  • Humans
  • Immunologic Memory / immunology*
  • Leukocyte Common Antigens / biosynthesis
  • T-Lymphocyte Subsets / chemistry
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism*
  • Tumor Necrosis Factor Receptor Superfamily, Member 7 / biosynthesis

Substances

  • Antigens, CD
  • CD57 Antigens
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Leukocyte Common Antigens