Fv2 Encodes a Truncated Form of the Stk Receptor Tyrosine Kinase

Nat Genet. 1999 Oct;23(2):159-65. doi: 10.1038/13787.

Abstract

The Friend virus susceptibility 2 (Fv2) locus encodes a dominant host factor that confers susceptibility to Friend virus-induced erythroleukaemia in mice. We mapped Fv2 to a 1.0-Mb interval that also contained the gene (Ron) encoding the stem cell kinase receptor (Stk). A truncated form of Stk (Sf-stk), which was the most abundant form of Stk in Fv2-sensitive (Fv2ss) erythroid cells, was not expressed in Fv2 resistant (Fv2rr) cells. Enforced expression of Sf-stk conferred susceptibility to Friend disease, whereas targeted disruption of Ron caused resistance. We conclude that the Fv2 locus encodes Ron, and that a naturally expressed, truncated form of Stk confers susceptibility to Friend virus-induced erythroleukaemia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • Contig Mapping
  • Friend murine leukemia virus*
  • Gene Expression
  • Genetic Predisposition to Disease
  • Leukemia, Erythroblastic, Acute / genetics*
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred CBA
  • Mice, Inbred NZB
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Muridae
  • Protein Isoforms / genetics
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptors, Cell Surface / genetics*
  • Retroviridae Infections / genetics
  • Species Specificity
  • Spleen / cytology
  • Spleen / metabolism
  • Spleen / pathology
  • Tumor Virus Infections / genetics

Substances

  • Protein Isoforms
  • Receptors, Cell Surface
  • RON protein
  • Receptor Protein-Tyrosine Kinases