Comparison of different primers for rapid detection of Salmonella using the polymerase chain reaction

Mol Cell Probes. 1999 Oct;13(5):341-7. doi: 10.1006/mcpr.1999.0257.

Abstract

Salmonella is the leading cause of food-borne diarrhoeas in the US. In recent years polymerase chain reaction (PCR) has become the method of choice for rapid and sensitive detection of Salmonellae in contaminated foods. As a result, several different primer sets have been reported for use in PCR-based assay systems. In order to identify an optimal primer set from among the wide range of primers reported in the literature, we synthesized five different pairs and evaluated their relative performance in PCR under uniform assay conditions using a common panel of the target (Salmonella) and non-target (non- Salmonella) bacterial strains. Of the five sets of primers tested, the one designed on the basis of a 199 bp repeat sequence of S. weltevreden[Jitrapakdee et al. (1995) Molecular and Cellular Probes 9, 375-382] gave optimal results with most bacterial strains examined.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Primers / standards*
  • DNA, Bacterial / analysis*
  • Polymerase Chain Reaction / instrumentation
  • Polymerase Chain Reaction / methods
  • Salmonella / genetics*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • DNA, Bacterial