Engineering the Mouse Genome by Site-Specific Recombination

Curr Opin Biotechnol. 1999 Oct;10(5):470-6. doi: 10.1016/s0958-1669(99)00012-9.


Site-specific recombination systems are powerful tools for introducing predetermined modifications into eukaryotic genomes. Recent advances allow the manipulation of chromosomal DNA in a spatially and temporally controlled manner in mice, offering unprecedented possibilities for studying mammalian genome function and for generating animal models for human diseases.

Publication types

  • Review

MeSH terms

  • Animals
  • Chromosomes
  • Gene Silencing
  • Genetic Engineering / methods
  • Genetic Vectors / genetics
  • Genome*
  • Integrases / genetics
  • Mice
  • Mice, Transgenic / genetics*
  • Mutagenesis
  • Mutagenesis, Site-Directed*
  • Recombination, Genetic*
  • Viral Proteins*
  • Viruses / genetics


  • Viral Proteins
  • Cre recombinase
  • Integrases