A gene family encoding glutathione peroxidase homologues in Hordeum vulgare (barley)

FEBS Lett. 1999 Oct 1;459(1):33-8. doi: 10.1016/s0014-5793(99)01208-9.

Abstract

We have isolated and characterised three barley cDNAs encoding glutathione peroxidase (GPX) homologues, designated HVGPH1, HVGPH2 and HVGPH3. HVGPH1 may represent a cytosolic form of GPX. The structure of the HVGPH2 N-terminal domain is typical for a plastid transit peptide. A potential peroxisomal targeting sequence occurs near the N-terminus of HVGPH3. Transcript levels of HVGPH1 and HVGPH2 were increased in leaves undergoing stress. In contrast, HVGPH3 mRNA accumulation showed a negative response to stress. Our data indicate that the barley genome bears a small gene family encoding GPX homologues differing in their function and cellular localisation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Complementary / analysis
  • Genes, Plant
  • Glutathione Peroxidase / genetics*
  • Hordeum / enzymology*
  • Hordeum / genetics*
  • Hordeum / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Glutathione Peroxidase

Associated data

  • GENBANK/AJ238697
  • GENBANK/AJ238744
  • GENBANK/AJ238745