A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit

FEBS Lett. 1999 Oct 1;459(1):69-74. doi: 10.1016/s0014-5793(99)01188-6.


We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants. Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-gammaS, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61beta, a subunit of the heterotrimeric protein conducting channel SEC61p. Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61beta in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61beta subunit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-Ribosylation Factors / biosynthesis
  • ADP-Ribosylation Factors / genetics*
  • ADP-Ribosylation Factors / isolation & purification
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Membrane Proteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger / biosynthesis
  • SEC Translocation Channels
  • Sequence Homology, Amino Acid
  • Subcellular Fractions
  • Tumor Cells, Cultured


  • Membrane Proteins
  • RNA, Messenger
  • SEC Translocation Channels
  • ADP-Ribosylation Factors
  • Arl6 protein, mouse

Associated data

  • GENBANK/AF031903
  • GENBANK/AF133669
  • GENBANK/AF133670
  • GENBANK/AF133910
  • GENBANK/AF133911
  • GENBANK/AF133912
  • GENBANK/AF133913