ade9 is an allele of SER1 and plays an indirect role in purine biosynthesis

Yeast. 1999 Sep 30;15(13):1347-55. doi: 10.1002/(SICI)1097-0061(19990930)15:13<1347::AID-YEA461>3.0.CO;2-A.


In this study we demonstrate that ade9 plays an indirect role in purine biosynthesis as a non-functional allele of SER1 in Saccharomyces cerevisiae. The SER1 locus, encoding 3-phosphoserine aminotransferase required for serine biosynthesis, is located on chromosome XV and resides approximately where ade9 had previously been mapped genetically. A minimal functional construct of SER1 is necessary and sufficient to complement both the adenine- and serine-requiring phenotypes of ade9 strains. In addition, adequate exogenous serine levels mask the adenine phenotype of ade9. A disruption of SER1 behaves in the same manner phenotypically as the original ade9 strain. Therefore, ade9 can be more accurately described as an allele of SER1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles*
  • Formates / pharmacology
  • Gene Expression Regulation, Fungal
  • Genes, Fungal*
  • Mutation
  • Purines / biosynthesis*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Serine / biosynthesis
  • Transaminases / genetics*


  • Formates
  • Purines
  • formic acid
  • Serine
  • Transaminases
  • phosphoserine aminotransferase