Reciprocal changes in the expression of neurotrophin mRNAs in target tissues and peripheral nerves of aged rats

Neurosci Lett. 1999 Oct 8;273(3):187-90. doi: 10.1016/s0304-3940(99)00655-2.

Abstract

trk receptors are downregulated in both dorsal root ganglion (DRG) and spinal motoneurons of aged rats with behavioral sensorimotor deficits. Here we provide evidence, using reverse transcription-polymerase chain reaction (RT-PCR), of decreased levels of neurotrophin (nerve growth factor, NGF; brain-derived neurotrophic factor, BDNF; neurotrophin-3, NT-3; and neurotrophin-4, NT-4) mRNAs in target muscles. Moreover, the degree of neurotrophin mRNA decrease in target muscles seems to co-vary with the extent of sensorimotor disturbances. In contrast, the peripheral nerve of aged rats showed a reciprocal regulation of neurotrophins, with increased levels of NGF, BDNF, and NT-4 mRNAs. Taken together, evidence suggest an aging-related attenuation of neurotrophin signaling between target tissues, on one hand, and DRG and motoneurons, on the other, and, furthermore, that target-derived neurotrophins regulate the expression levels of trk mRNAs in both DRG and motoneurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Ganglia, Spinal / metabolism*
  • Motor Neurons / metabolism*
  • Nerve Growth Factors / genetics
  • Nerve Growth Factors / metabolism*
  • Peripheral Nerves / metabolism
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sciatic Nerve / metabolism*
  • Spinal Cord / metabolism

Substances

  • Nerve Growth Factors
  • RNA, Messenger