Rescue of influenza A virus from recombinant DNA

J Virol. 1999 Nov;73(11):9679-82. doi: 10.1128/JVI.73.11.9679-9682.1999.


We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • DNA, Recombinant / genetics*
  • Genetic Techniques
  • Humans
  • Influenza A virus / genetics*
  • Influenza A virus / physiology*
  • Plasmids / genetics
  • RNA, Viral / metabolism
  • Transcription, Genetic
  • Transfection
  • Viral Proteins / metabolism


  • DNA, Recombinant
  • RNA, Viral
  • Viral Proteins