Application of a time-resolved fluoroimmunoassay for the analysis of normal prion protein in human blood and its components

Vox Sang. 1999;77(2):88-96. doi: 10.1159/000031082.


Background and objectives: To quantify the cellular isoform of prion protein (PrP(c)) in human blood using a new time-resolved dissociation-enhanced fluoroimmunoassay (DELFIA).

Materials and methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP(c) were analysed.

Results: 26. 5% of blood PrP(c) was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations).

Conclusion: The majority of blood PrP(c) is found in the platelet and plasma compartments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens
  • Blood Platelets / chemistry
  • Drug Stability
  • Erythrocytes / chemistry
  • Fluoroimmunoassay / methods
  • Humans
  • Leukocytes, Mononuclear / chemistry
  • Neutrophils / chemistry
  • Plasma / chemistry
  • Prions / blood*
  • Prions / chemistry
  • Prions / immunology


  • Antigens
  • Prions