A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption

Microbiology. 1999 Sep;145 ( Pt 9):2415-2422. doi: 10.1099/00221287-145-9-2415.

Abstract

A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp, designated CaFET3, was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70.5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55%. CaFET3 was localized on chromosome 6. A null mutant (fet3delta/fet3delta) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wild-type strain the fet3delta mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3delta mutant in iron-limited medium was compensated by transformation with CaFET3. The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Candida albicans / enzymology
  • Candida albicans / genetics*
  • Candida albicans / pathogenicity
  • Candidiasis / microbiology*
  • Cloning, Molecular
  • Copper / metabolism*
  • Gene Deletion
  • Genes, Fungal
  • Genetic Complementation Test
  • Humans
  • Iron / metabolism
  • Mice
  • Molecular Sequence Data
  • Oxidoreductases / chemistry
  • Oxidoreductases / genetics*
  • Oxidoreductases / metabolism*
  • Plasmids / genetics
  • Polymerase Chain Reaction / methods
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Sequence Analysis, DNA
  • Transformation, Genetic

Substances

  • Copper
  • Iron
  • Oxidoreductases

Associated data

  • GENBANK/Y09329