A Xenopus protein related to hnRNP I has a role in cytoplasmic RNA localization

Mol Cell. 1999 Sep;4(3):431-7. doi: 10.1016/s1097-2765(00)80345-7.

Abstract

Cytoplasmic localization of mRNA molecules is a powerful mechanism for generating cell polarity. In vertebrates, one paradigm is localization of Vg1 RNA within the Xenopus oocyte, a process directed by recognition of a localization element within the Vg1 3' UTR. We show that specific base changes within the localization element abolish both localization in vivo and binding in vitro by a single protein, VgRBP60. VgRBP60 is homologous to a human hnRNP protein, hnRNP I, and combined immunolocalization and in situ hybridization demonstrate striking colocalization of hnRNP I and Vg1 RNA within the vegetal cytoplasm of the Xenopus oocyte. These results implicate a novel role in cytoplasmic RNA transport for this family of nuclear RNA-binding proteins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Biological Transport
  • Cell Compartmentation
  • Cell Polarity
  • Cloning, Molecular
  • Cytoplasm / chemistry
  • Glycoproteins / genetics*
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics
  • Nuclear Proteins / isolation & purification*
  • Nuclear Proteins / metabolism
  • Oocytes / chemistry
  • Polypyrimidine Tract-Binding Protein
  • Protein Binding
  • RNA, Messenger / isolation & purification*
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / isolation & purification*
  • RNA-Binding Proteins / metabolism
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / isolation & purification*
  • Ribonucleoproteins / metabolism
  • Transforming Growth Factor beta
  • Xenopus
  • Xenopus Proteins

Substances

  • 3' Untranslated Regions
  • GDF1 protein, Xenopus
  • Glycoproteins
  • Nuclear Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Transforming Growth Factor beta
  • Xenopus Proteins
  • Polypyrimidine Tract-Binding Protein

Associated data

  • GENBANK/AF091370