Characterization of an alpha-tubulin gene of Cryptosporidium parvum

J Eukaryot Microbiol. Sep-Oct 1999;46(5):545-7. doi: 10.1111/j.1550-7408.1999.tb06073.x.

Abstract

A gene encoding an alpha-tubulin of Cryptosporidium parvum was isolated and characterized. It had no introns, and encoded a 441-amino acid protein whose predicted ORF represented a typical alpha-tubulin protein with a MW of 50.5 kDa. This tubulin had an amino acid sequence similarity with Apicomplexa Plasmodium falciparum and Toxoplasma gondii higher than 88% and shared a number of conserved motifs.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blotting, Western
  • Cryptosporidium parvum / genetics*
  • DNA, Protozoan / genetics
  • Genes, Protozoan*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA, Protozoan / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA
  • Tubulin / genetics*
  • Tubulin / metabolism*

Substances

  • DNA, Protozoan
  • RNA, Protozoan
  • Tubulin

Associated data

  • GENBANK/AF082877