Organization of DNA replication origins in the fission yeast genome

EMBO J. 1999 Oct 15;18(20):5683-90. doi: 10.1093/emboj/18.20.5683.


Eukaryotic DNA replication initiates at multiple points along the chromosomes known as replication origins (ORIs). We have developed a strategy to identify ORIs directly from replication intermediates in the fission yeast Schizosaccharomyces pombe. Mapping of a selection of the novel ORIs onto the genome reveals their preferential localization at intergenic regions upstream from genes. These results are supported by the observation that a large proportion of regions overlapping gene promoters contain active ORIs. Mapping of the genomic ars1 replication origin at nucleotide resolution shows that replication initiates at a defined position immediately upstream from the hus5(+) promoter. Deletion analysis indicates that the regulatory elements required to initiate transcription and replication lie in close proximity, suggesting a possible relationship between both processes in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • DNA Primers / genetics
  • DNA Replication / genetics*
  • DNA, Fungal / genetics
  • Genome, Fungal*
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Replication Origin*
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism*
  • Transcription, Genetic


  • DNA Primers
  • DNA, Fungal