The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries

Gene. 1999 Sep 3;237(1):61-70. doi: 10.1016/s0378-1119(99)00302-9.


Metalloproteinase-disintegrins (ADAMs) are membrane-spanning multi-domain proteins containing a zinc metalloproteinase domain and a disintegrin domain which may serve as an integrin ligand. Based on a conserved sequence within the disintegrin domain, GE(E/Q)CDCG, seven genes were isolated from a human genomic library. Two of these genes lack introns and show testis-specific expression (ADAM20 and ADAM21), while the other two genes contain introns (ADAM22 and ADAM23) and are expressed predominantly in the brain. In addition, three pseudogenes were isolated; one of which evolved from ADAM21. Human chromosomal mapping indicated that ADAM22 and ADAM23 mapped to chromosome 7q21 and 2q33, respectively, while the three pseudogenes 1-2, 3-3, and 1-32 mapped to chromosome 14q24.1, 8p23, and 14q24.1, respectively. An ancestral analysis of all known ADAMs indicates that the zinc-binding motif in the catalytic domain arose once in a common ancestor and was lost by those members lacking this motif.

MeSH terms

  • ADAM Proteins
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosomes, Human*
  • Conserved Sequence
  • Disintegrins / genetics*
  • Evolution, Molecular
  • Genomic Library
  • Humans
  • Introns
  • Membrane Proteins*
  • Metalloendopeptidases / genetics*
  • Metalloendopeptidases / metabolism
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Oligonucleotides / genetics
  • Phylogeny*
  • Pseudogenes
  • Sequence Homology, Amino Acid


  • Disintegrins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Oligonucleotides
  • ADAM Proteins
  • ADAM20 protein, human
  • ADAM21 protein, human
  • ADAM22 protein, human
  • Adam23 protein, mouse
  • Metalloendopeptidases

Associated data

  • GENBANK/AF158637
  • GENBANK/AF158638
  • GENBANK/AF158639
  • GENBANK/AF158640
  • GENBANK/AF158641
  • GENBANK/AF158642
  • GENBANK/AF158643
  • GENBANK/AF158644