Sequence analysis of the chitin synthase A gene of the Dutch elm pathogen Ophiostoma novo-ulmi indicates a close association with the human pathogen Sporothrix schenckii

Gene. 1999 Sep 3;237(1):215-21. doi: 10.1016/s0378-1119(99)00291-7.

Abstract

Degenerate oligonucleotide primers were designed according to conserved regions of the chitin synthase gene family and used to amplify a 621 basepair (bp) fragment from genomic DNA of Ophiostoma novo-ulmi, the causal agent of Dutch elm disease. The amplification product was used as a hybridization probe to screen a library of genomic DNA sequences and to retrieve a full-length chitin synthase gene (chsA). The putative coding region of the gene was 2619 bp long, lacked introns, and encoded a polypeptide of 873 amino acids. Based on the similarity of the predicted amino acid sequence to the full-length chsC gene of Aspergillus nidulans and chsA gene of Ampelomyces quisqualis, the O. novo-ulmi chsA was classified as a Class I chitin synthase. The phylogenies constructed, according to a subregion of all available chitin synthases, showed that O. novo-ulmi consistently clustered most closely with the human pathogen Sporothrix schenckii, recently classified as a member of the mitosporic Ophiostomataceae. Disruption of the chsA gene locus had no obvious effects on the growth or morphology of the fungus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Ascomycota / genetics*
  • Chitin Synthase / genetics*
  • Chitin Synthase / metabolism
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Phylogeny
  • Plant Diseases / microbiology
  • Sequence Homology, Amino Acid
  • Sporothrix / genetics*
  • Sporothrix / pathogenicity
  • Trees / microbiology*

Substances

  • Chitin Synthase

Associated data

  • GENBANK/AF157477