Monophosphoryl lipid A (MPL) formulations for the next generation of vaccines

Methods. 1999 Sep;19(1):103-7. doi: 10.1006/meth.1999.0834.


Many of the latest trends in vaccine development are dependent on immunological adjuvants that mediate and promote a wide variety of immune responses. One promising adjuvant candidate, monophosphoryl lipid A (MPL) immunostimulant, is being investigated with many of these new vaccine approaches in either preclinical or clinical trials. This is possible because different vehicle formulations can significantly influence the type of immunological response MPL promotes. Procedures are provided for formulating MPL in an aqueous vehicle or an oil-in-water emulsion. These two MPL formulations can be beneficial for most vaccine approaches being investigated today.

Publication types

  • Review

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Adjuvants, Immunologic / isolation & purification
  • Animals
  • Antigens / administration & dosage
  • Drug Compounding
  • Emulsions
  • Lipid A / administration & dosage
  • Lipid A / analogs & derivatives*
  • Lipid A / isolation & purification
  • Mice
  • Oils
  • Vaccines / administration & dosage*
  • Water


  • Adjuvants, Immunologic
  • Antigens
  • Emulsions
  • Lipid A
  • Oils
  • Vaccines
  • Water
  • monophosphoryl lipid A