Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

Brain Res. 1999 Sep 18;842(1):211-4. doi: 10.1016/s0006-8993(99)01818-1.

Abstract

In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Apoptosis / drug effects
  • Caspase 3
  • Caspases / biosynthesis
  • Cerebrovascular Circulation / drug effects
  • DNA Fragmentation / drug effects
  • Enzyme Induction / drug effects
  • HSP72 Heat-Shock Proteins
  • Heat-Shock Proteins / biosynthesis
  • Immunohistochemistry
  • In Situ Nick-End Labeling
  • Infarction, Middle Cerebral Artery / drug therapy*
  • Infarction, Middle Cerebral Artery / pathology
  • Infarction, Middle Cerebral Artery / physiopathology
  • Ischemic Attack, Transient / drug therapy*
  • Ischemic Attack, Transient / pathology
  • Ischemic Attack, Transient / physiopathology
  • Middle Cerebral Artery / physiology*
  • Neurotrophin 3 / administration & dosage
  • Neurotrophin 3 / therapeutic use*
  • Rats

Substances

  • HSP72 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Neurotrophin 3
  • Casp3 protein, rat
  • Caspase 3
  • Caspases