Localization of opioid-binding cell adhesion molecule (OBCAM) in adult rat brain

Brain Res. 1999 Sep 25;842(2):482-6. doi: 10.1016/s0006-8993(99)01831-4.


We investigated the tissue distribution and brain localization of opioid-binding cell adhesion molecule (OBCAM) in the adult rats by immunoblotting and immunohistochemistry using a monoclonal anti-OBCAM peptide antibody that is specific for OBCAM. OBCAM was preferentially expressed in the central nervous system (CNS) and at a very low level in the spleen. Within the brain, OBCAM was distributed in almost all the gray matter, but little or no immunoreactive OBCAM was found in the white matter. Morphologically, the distribution pattern of OBCAM immunoreactivity was very similar to that of synaptophysin, suggesting a role in the synaptic machinery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Specificity
  • Brain / cytology*
  • Brain Chemistry*
  • Carrier Proteins / analysis*
  • Cell Adhesion Molecules / analysis*
  • Cell Membrane / chemistry
  • Cell Membrane / ultrastructure
  • GPI-Linked Proteins
  • Immunoblotting
  • Immunohistochemistry
  • Nerve Tissue Proteins / analysis
  • Organ Specificity
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord / chemistry
  • Spinal Cord / cytology
  • Synaptophysin / analysis


  • Carrier Proteins
  • Cell Adhesion Molecules
  • GPI-Linked Proteins
  • Nerve Tissue Proteins
  • Opcml protein, rat
  • Synaptophysin