Presence and expression of a novel variant form of ST2 gene product in human leukemic cell line UT-7/GM

Biochem Biophys Res Commun. 1999 Oct 14;264(1):14-8. doi: 10.1006/bbrc.1999.1469.

Abstract

A novel variant cDNA from the human ST2 gene other than ST2 or ST2L was identified and tentatively named ST2V. Alternative splicing inserts a new exon which leads to a change in the C-terminal portion of ST2, causing it to gain a hydrophobic tail instead of losing the third immunoglobulin-like domain. ST2V is expressed in human leukemic cell line UT-7 and its sublines UT-7/GM, UT-7/EPO, and UT-7/TPO, in addition to human helper T cell line 5C10. The amount of ST2V mRNA is greatly diminished when UT-7/GM cells are induced to differentiate into either erythroblastic or megakaryoblastic phenotypes. The possible roles of the ST2V in growth and differentiation are intriguing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • Cell Differentiation / genetics
  • DNA, Complementary / analysis
  • Exons
  • Gene Library
  • Genetic Variation*
  • Genome, Human
  • Humans
  • Interleukin-1 Receptor-Like 1 Protein
  • Introns
  • Leukemia
  • Membrane Proteins*
  • Molecular Sequence Data
  • Protein Biosynthesis
  • Proteins / genetics*
  • Receptors, Cell Surface
  • T-Lymphocytes, Helper-Inducer
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • IL1RL1 protein, human
  • Interleukin-1 Receptor-Like 1 Protein
  • Membrane Proteins
  • Proteins
  • Receptors, Cell Surface

Associated data

  • GENBANK/AB029084