Kinetics and interactions of molybdenum and iron-sulfur centers in bacterial enzymes of the xanthine oxidase family: mechanistic implications

Biochemistry. 1999 Oct 19;38(42):14077-87. doi: 10.1021/bi991089s.

Abstract

For isoquinoline 1-oxidoreductase (IsoOr), the reaction mechanism under turnover conditions was studied by EPR spectroscopy using rapid-freeze methods. IsoOr displays several EPR-active Mo(V) species including the "very rapid" component found also in xanthine oxidase (XanOx). For IsoOr, unlike XanOx or quinoline 2-oxidoreductase (QuinOr), this species is stable for about 1 h in the absence of an oxidizing substrate [Canne, C., Stephan, I., Finsterbusch, J., Lingens, F., Kappl, R., Fetzner, S., and Hüttermann, J. (1997) Biochemistry 36, 9780-9790]. Under rapid-freeze conditions in the presence of ferricyanide the very rapid species behaves as a kinetically competent intermediate present only during steady-state turnover. To explain the persistence of the very rapid species in IsoOr in the absence of an added oxidant, extremely slow product dissociation is required. This new finding that oxidative conditions facilitate decay of the very rapid signal for IsoOr supports the mechanism of substrate turnover proposed by Lowe, Richards, and Bray [Lowe, D. J., Richards, R. L., and Bray, R. C. (1997) Biochem. Soc. Trans. 25, 774-778]. Additional stopped-flow data reveal that alternative catalytic cycles occur in IsoOr and show that the product dissociates after transfer of a single oxidizing equivalent from ferricyanide. In rapid-freeze measurements magnetic interactions of the very rapid Mo(V) species and the iron-sulfur center FeSI of IsoOr and QuinOr were observed, proving that FeSI is located close to the molybdopterin cofactor in the two proteins. This finding is used to relate the two different iron-sulfur centers of the aldehyde oxidoreductase structure with the EPR-detectable FeS species of the enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anisotropy
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism*
  • Coenzymes*
  • Electron Spin Resonance Spectroscopy / methods
  • Iron-Sulfur Proteins / chemistry
  • Iron-Sulfur Proteins / metabolism*
  • Kinetics
  • Metalloproteins / chemistry
  • Metalloproteins / metabolism*
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / metabolism
  • Molybdenum Cofactors
  • Oxidation-Reduction
  • Oxidoreductases / chemistry
  • Oxidoreductases / metabolism
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Pteridines / chemistry
  • Pteridines / metabolism*
  • Spectrophotometry / methods
  • Structure-Activity Relationship
  • Substrate Specificity
  • Xanthine Oxidase / chemistry
  • Xanthine Oxidase / metabolism*

Substances

  • Bacterial Proteins
  • Coenzymes
  • Iron-Sulfur Proteins
  • Metalloproteins
  • Molybdenum Cofactors
  • Pteridines
  • molybdenum cofactor
  • Mixed Function Oxygenases
  • Oxidoreductases
  • Xanthine Oxidase
  • Oxidoreductases Acting on CH-CH Group Donors
  • isoquinoline 1-oxidoreductase
  • quinaldine oxidoreductase
  • quinoline 2-oxidoreductase