KIAA0369, doublecortin-like kinase, is expressed during brain development

J Neurosci Res. 1999 Nov 15;58(4):567-75.

Abstract

During embryonic development, the cerebral cortex attains its characteristic adult laminated structure. The finding that X-linked lissencephaly patients harbor mutations in the doublecortin gene implicated this gene product in the process of corticogenesis. An autosomal human gene, KIAA0369, with a high level of similarity to doublecortin, has been cloned from human adult brain. This gene product contains a kinase domain in addition to a doublecortin-like domain. In order to evaluate whether this doublecortin-like kinase also plays a role during brain development, we cloned and studied the expression pattern of the mouse homolog. Three cDNA products of this gene were cloned: one, doublecortin-like kinase, the second containing only the doublecortin-like region, and the third containing only the kinase domain, a homolog of the previously cloned rat CPG16 gene. We studied doublecortin-like kinase expression in mouse using Northern blot analysis, in situ hybridization, and Western blot analysis, and conclude that doublecortin-like kinase is expressed in multiple regions of embryonic brain including the developing cerebral cortex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Brain / embryology
  • Brain / enzymology*
  • Brain / growth & development*
  • Calcium-Calmodulin-Dependent Protein Kinases / biosynthesis*
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Cells, Cultured
  • Cloning, Molecular
  • Gene Library
  • Humans
  • In Situ Hybridization
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Plasmids / genetics
  • Protein-Serine-Threonine Kinases*
  • RNA Probes
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection

Substances

  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • RNA Probes
  • Dcamkl1 protein, mouse
  • Dclk1 protein, rat
  • DCLK1 protein, human
  • Protein-Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases

Associated data

  • GENBANK/AF155819
  • GENBANK/AF155820
  • GENBANK/AF155821