GFP-tagged Balancer Chromosomes for Drosophila Melanogaster

Mech Dev. 1999 Nov;88(2):229-32. doi: 10.1016/s0925-4773(99)00174-4.

Abstract

We constructed green fluorescent protein (GFP)-expressing balancer chromosomes for each of the three major chromosomes of Drosophila melanogaster. Expression of GFP in these chromosomes is driven indirectly by a Kruppel (Kr) promoter, via the yeast GAL4-UAS regulatory system. GFP fluorescence can be seen in embryos as early as the germ band extension stage, and can also be seen in larvae, pupae, and adults. We show the patterns of GFP expression of these balancers and demonstrate the use of the balancers to identify homozygous progeny.

MeSH terms

  • Animals
  • Chromosomes / genetics*
  • DNA-Binding Proteins / genetics
  • Drosophila Proteins
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Embryo, Nonmammalian
  • Female
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins
  • Kruppel-Like Transcription Factors
  • Luminescent Proteins / genetics*
  • Male
  • Promoter Regions, Genetic
  • Repressor Proteins*
  • Transcription Factors / genetics

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • Kr protein, Drosophila
  • Kruppel-Like Transcription Factors
  • Luminescent Proteins
  • Repressor Proteins
  • Transcription Factors
  • Green Fluorescent Proteins