The human REV1 gene codes for a DNA template-dependent dCMP transferase

Nucleic Acids Res. 1999 Nov 15;27(22):4468-75. doi: 10.1093/nar/27.22.4468.


DNA is frequently damaged by various physical and chemical agents. DNA damage can lead to mutations during replication. In the yeast Saccharomyces cerevisiae, the damage-induced mutagenesis pathway requires the Rev1 protein. We have isolated a human cDNA homologous to the yeast REV1 gene. The human REV1 cDNA consists of 4255 bp and codes for a protein of 1251 amino acid residues with a calculated molecular weight of 138 248 Da. The human REV1 gene is localized between 2q11.1 and 2q11.2. We show that the human REV1 protein is a dCMP transferase that specifically inserts a dCMP residue opposite a DNA template G. In addition, the human REV1 transferase is able to efficiently and specifically insert a dCMP opposite a DNA template apurinic/apyrimidinic (AP) site or a uracil residue. These results suggest that the REV1 transferase may play a critical role during mutagenic translesion DNA synthesis bypassing a template AP site in human cells. Consistent with its role as a fundamental mutagenic protein, the REV1 gene is ubiquitously expressed in various human tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 2
  • Cloning, Organism
  • Conserved Sequence
  • DNA, Complementary / analysis
  • Deoxycytidine Monophosphate / metabolism
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics*
  • Humans
  • Molecular Sequence Data
  • Nucleotidyltransferases*
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Templates, Genetic


  • DNA, Complementary
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Deoxycytidine Monophosphate
  • Nucleotidyltransferases
  • REV1 protein, S cerevisiae

Associated data

  • GENBANK/AF151538
  • GENBANK/AF153594