The assignment of chemokine-chemokine receptor pairs: TARC and MIP-1 beta are not ligands for human CC-chemokine receptor 8

Eur J Immunol. 1999 Oct;29(10):3210-5. doi: 10.1002/(SICI)1521-4141(199910)29:10<3210::AID-IMMU3210>3.0.CO;2-W.

Abstract

Identification of chemokine receptors and their associated ligands is crucial to the understanding of most immune reactions. Three human chemokines [I-309, thymus and activation-regulated chemokine (TARC) and macrophage inflammatory protein-1beta (MIP-1beta)] have been reported to be ligands for CC-chemokine receptor 8 (CCR8). In this report, we present evidence that TARC and MIP-1beta did not bind to or induce chemotaxis through CCR8 on a stable transfected cell line (1D-21) and did not bind to CCR8 on in vitro differentiated human CD4(+) Th(2) cell cultures. Also, I-309-dependent calcium mobilization in 1D-21 cells and in Th(2) cells was desensitized by I-309 but not by MIP-1beta or TARC. These results provide strong evidence that, at physiologically relevant concentrations, I-309 is the only known human ligand for CCR8. These data also provide a framework for suggesting minimum requirements for the assignment of chemokine receptor-ligand pairs.

MeSH terms

  • CD4 Antigens / biosynthesis
  • CD4 Antigens / genetics
  • Calcium / metabolism
  • Cells, Cultured
  • Chemokine CCL17
  • Chemokine CCL4
  • Chemokines, CC / immunology
  • Chemokines, CC / metabolism*
  • Chemotaxis, Leukocyte / immunology
  • DNA / genetics
  • Humans
  • Ligands
  • Macrophage Inflammatory Proteins / immunology
  • Macrophage Inflammatory Proteins / metabolism*
  • Receptors, CCR8
  • Receptors, Chemokine / biosynthesis
  • Receptors, Chemokine / genetics
  • Receptors, Chemokine / immunology
  • Receptors, Chemokine / metabolism*
  • Th2 Cells / immunology
  • Th2 Cells / metabolism
  • Time Factors
  • Transfection

Substances

  • CCL17 protein, human
  • CCR8 protein, human
  • CD4 Antigens
  • Chemokine CCL17
  • Chemokine CCL4
  • Chemokines, CC
  • Ligands
  • Macrophage Inflammatory Proteins
  • Receptors, CCR8
  • Receptors, Chemokine
  • DNA
  • Calcium