A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1)

Curr Genet. 1999 Oct;36(4):195-200. doi: 10.1007/s002940050490.


The HAP1 gene encodes a complex transcriptional regulator of many genes involved in electron-transfer reactions and is essential in anaerobic or heme-depleted conditions. We show here that strains derived from S288c carry a defective Ty1 element inserted in the 3' region of the HAP1 ORF. This mutant allele acts as a HAP1 null allele in terms of cytochrome c expression and CYC1 UAS1-dependent transcription, but is able to sustain limited growth in heme-depleted conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytochrome c Group / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Mutagenesis, Insertional
  • Retroelements
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription Factors


  • Cytochrome c Group
  • DNA-Binding Proteins
  • Fungal Proteins
  • HAP1 protein, S cerevisiae
  • Retroelements
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors