Characterization and DNA-binding properties of GRF, a novel monomeric binding orphan receptor related to GCNF and betaFTZ-F1

Eur J Biochem. 1999 Nov;266(1):181-90. doi: 10.1046/j.1432-1327.1999.00842.x.

Abstract

A PCR approach has been used to isolate, from Bombyx mori, a cDNA encoding a novel orphan receptor (GRF) that is most closely related to Bombyx betaFTZ-F1 and to the vertebrate germ cell nuclear factor. The major GRF mRNA is detected in most tissues as an 8-kb transcript whose amount follows the circulating ecdysteroid concentration with a delay. The expression pattern of GRF is similar to that of the Bombyx homologue of the Drosophila early-late gene DHR3, and precedes that of betaFTZ-F1 in all stages and tissues examined. The GRF protein is thus likely to be required in many tissues, but in a temporally restricted manner suggesting that GRF has a well-defined function in the ecdysteroid-induced transcription cascade. The GRF protein binds in vitro to a single oestrogen receptor half-site AGGTCA preceded by a 5'-TCA extension, and is therefore a potential co-regulator of the orphan receptors betaFTZ-F1 and DHR39.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Bombyx / chemistry*
  • Bombyx / growth & development
  • Cloning, Molecular
  • DNA / metabolism*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • Ecdysterone / pharmacology
  • Fushi Tarazu Transcription Factors
  • Gene Expression Regulation, Developmental / drug effects
  • Genes, Insect*
  • Homeodomain Proteins
  • Insect Proteins / genetics
  • Insect Proteins / isolation & purification*
  • Insect Proteins / metabolism
  • Molecular Sequence Data
  • Nuclear Receptor Subfamily 6, Group A, Member 1
  • Protein Structure, Tertiary
  • Receptors, Cytoplasmic and Nuclear / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Steroidogenic Factor 1
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • Insect Proteins
  • Nuclear Receptor Subfamily 6, Group A, Member 1
  • Receptors, Cytoplasmic and Nuclear
  • Recombinant Fusion Proteins
  • Steroidogenic Factor 1
  • Transcription Factors
  • nuclear hormone receptor FTZ-F1, Bombyx mori
  • Ecdysterone
  • DNA

Associated data

  • GENBANK/AF124981