Gene transfer in the gastrointestinal tract

Appl Environ Microbiol. 1999 Nov;65(11):5139-41. doi: 10.1128/AEM.65.11.5139-5141.1999.


The maximum in vivo transfer rate of plasmid pAMbeta1 in the gut was 0.03 transconjugant per recipient cell, and this rate could be simulated in vitro only by forced filter mating. Transfer was not detected in liquid culture matings. Our findings demonstrate that in vitro methods, such as forced filter mating and liquid mating, underestimate the in vivo rates of gene transfer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacillaceae / genetics
  • Cellulase / genetics
  • Chickens
  • Conjugation, Genetic*
  • Digestive System / drug effects
  • Digestive System / microbiology*
  • Enterococcus faecium / genetics*
  • Gene Transfer Techniques*
  • Plasmids*
  • Probiotics / administration & dosage
  • Probiotics / pharmacology


  • Cellulase