Nuclear F-actin is required for AcMNPV nucleocapsid morphogenesis

Virology. 1999 Nov 10;264(1):1-4. doi: 10.1006/viro.1999.0008.


During nucleocapsid assembly, filamentous actin (F-actin) colocalizes with the major capsid protein of Autographa californica M nucleopolyhedrovirus (AcMNPV) within nuclei of infected lepidopteran host cells. Cytochalasin D (CD) disrupts actin filaments and prevents assembly of progeny AcMNPV, suggesting that nuclear F-actin is essential for nucleocapsid morphogenesis. Direct proof for this hypothesis was provided by the demonstration that two AcMNPV recombinants engineered to express either wild-type- or CD-resistant actin at equivalent rates were differentially sensitive to CD. The AcMNPV requirement for nuclear F-actin is unique among intracellular pathogens and may constitute a significant host range factor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Actins / drug effects
  • Actins / genetics
  • Actins / metabolism*
  • Animals
  • Cell Nucleus / physiology*
  • Cell Nucleus / ultrastructure
  • Cytochalasin D / pharmacology
  • Kinetics
  • Lepidoptera / virology
  • Morphogenesis
  • Nucleocapsid / biosynthesis*
  • Nucleopolyhedroviruses / genetics
  • Nucleopolyhedroviruses / physiology*
  • Recombinant Proteins / biosynthesis


  • Actins
  • Recombinant Proteins
  • Cytochalasin D