A novel human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, GalNAc-T7, with specificity for partial GalNAc-glycosylated acceptor substrates

FEBS Lett. 1999 Oct 29;460(2):226-30. doi: 10.1016/s0014-5793(99)01268-5.


A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partially GalNAc-glycosylated acceptor substrates. GalNAc-T7 showed no activity with a large panel of non-glycosylated peptides, but was selectively activated by partial GalNAc glycosylation of peptide substrates derived from the tandem repeats of human MUC2 and rat submaxillary gland mucin. The function of GalNAc-T7 is suggested to be as a follow-up enzyme in the initiation step of O-glycosylation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Glycosylation
  • Humans
  • Isoenzymes
  • Kinetics
  • Molecular Sequence Data
  • N-Acetylgalactosaminyltransferases / isolation & purification
  • N-Acetylgalactosaminyltransferases / metabolism*
  • Peptides / chemistry
  • Peptides / metabolism*
  • Rats
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Substrate Specificity
  • Tissue Distribution
  • Uridine Diphosphate / chemistry
  • Uridine Diphosphate / metabolism*


  • DNA, Complementary
  • Isoenzymes
  • Peptides
  • Recombinant Proteins
  • Uridine Diphosphate
  • N-Acetylgalactosaminyltransferases
  • UDP-N-acetylgalactosamine--polypeptide N-acetylgalactosaminyltransferase 7

Associated data

  • GENBANK/AJ002744