Porcine pulmonary surfactant preparations contain the antibacterial peptide prophenin and a C-terminal 18-residue fragment thereof

FEBS Lett. 1999 Oct 29;460(2):257-62. doi: 10.1016/s0014-5793(99)01363-0.


Surfactant preparations obtained from porcine lungs by extraction with chloroform/methanol followed by chromatography over Lipidex-5000 are used for treatment of respiratory distress syndrome in preterm infants. These preparations contain about 98% phospholipids and 1-2% of the hydrophobic pulmonary surfactant-associated proteins B and C (SP-B and SP-C). Separation of the proteins in the surfactant preparation by reversed-phase high performance liquid chromatography revealed, in addition to SP-B and SP-C, the presence of three peptides derived from the cathelicidin family of antibacterial peptides. The 79-residue proline-rich peptide prophenin (identical to that isolated from leukocytes), an 80-residue prophenin with an N-terminal pyroglutamic acid residue, and a C-terminal 18-residue fragment of prophenin were found in approximate molar ratios of 1:20:5. A synthetic version of the C-terminal 18-residue peptide exhibits salt-dependent antibacterial activity (higher activity in the absence of salt) against the Gram-positive bacterium Bacillus megaterium Bm11 and, to a lesser extent, against Gram-negative Escherichia coli D21 cells. It appears possible that the presence of prophenin peptides may contribute to the antibacterial properties of surfactant preparations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Infective Agents / analysis*
  • Antimicrobial Cationic Peptides
  • Chromatography, High Pressure Liquid
  • Lung / metabolism
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptides / analysis
  • Proteins / analysis*
  • Pulmonary Surfactants / metabolism*
  • Pyrrolidonecarboxylic Acid / analysis
  • Swine
  • Time Factors


  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • PF-2 protein, Sus scrofa
  • Peptides
  • Proteins
  • Pulmonary Surfactants
  • prophenin 1
  • Pyrrolidonecarboxylic Acid