Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-sigma (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas

Electrophoresis. 1999 Oct;20(14):2952-60. doi: 10.1002/(SICI)1522-2683(19991001)20:14<2952::AID-ELPS2952>3.0.CO;2-H.


In order to study possible mechanisms leading to chemoresistance in pancreatic adenocarcinoma we examined the global protein expression of pancreatic cancer cells in vitro. We used a cell culture model derived from the adenocarcinoma of the pancreas (EPP85-181P). A classical multidrug-resistant subline, EPP85-181RDB, selected in presence of daunorubicin, and an atypical multidrug-resistant cell variant, EPP85-181RNOV, selected in presence of mitoxantrone, were analyzed using two-dimensional electrophoresis. After staining and image analysis, spots of interest were isolated using preparative two-dimensional electrophoresis and subjected to mass spectrometry and microsequencing. Three proteins, E-FABP, cofilin, and 14-3-3-sigma (stratifin), were overexpressed in chemoresistant cell lines. Cofilin was present in both multidrug in chemoresistant cell lines. Cofilin was present in both multidrug-resistant cell lines. E-FABP and 14-3-3-sigma (stratifin) was found to be overexpressed only in the mitoxantrone-selected atypical multidrug-resistant cell line. The possible significance of these findings is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins
  • Actin Depolymerizing Factors
  • Adenocarcinoma / metabolism*
  • Biomarkers, Tumor*
  • Drug Resistance, Neoplasm
  • Electrophoresis, Gel, Two-Dimensional
  • Exonucleases*
  • Exoribonucleases
  • Humans
  • Mass Spectrometry
  • Microfilament Proteins / biosynthesis*
  • Neoplasm Proteins*
  • Pancreatic Neoplasms / metabolism*
  • Protein Biosynthesis*
  • Tumor Cells, Cultured


  • 14-3-3 Proteins
  • Actin Depolymerizing Factors
  • Biomarkers, Tumor
  • Microfilament Proteins
  • Neoplasm Proteins
  • Exonucleases
  • Exoribonucleases
  • SFN protein, human