This in vivo study demonstrated the effects of transducer frequency and output power on the sonographic contrast produced by Optison during continuous ultrasonographic imaging in both fundamental and harmonic modes. During fundamental imaging, Optison produced greater contrast intensity while imaging at higher transducer frequencies. However, in the harmonic mode, lower imaging frequencies caused Optison to produce more intense myocardial enhancement. Regardless of imaging mode, increases in transducer output power caused decreases in both contrast intensity and contrast duration produced by Optison. Proper selection of transducer frequency and output power will help optimize the quality and duration of contrast produced by Optison during continuous ultrasonographic imaging.