Genetic evidence for distinct roles of COX-1 and COX-2 in the immediate and delayed phases of prostaglandin synthesis in mast cells

Biochem Biophys Res Commun. 1999 Nov;265(1):205-10. doi: 10.1006/bbrc.1999.1658.


Activation of mast cells by aggregation of their high-affinity IgE receptors stimulates prostaglandin (PG) D(2) synthesis and secretion. An immediate phase of PGD(2) synthesis, complete within 30 min, is followed by a delayed, second phase of PGD(2) production that reaches a maximum 4 to 8 h after activation. Activation of mast cells from COX-2 (-/-) mice stimulates the release of PGD(2) during the first 30 min, whereas activation of mast cells from COX-1 (-/-) mice does not generate any PGD(2) in the first 2 h. On the other hand, COX-2 (-/-) cells do not participate in delayed phase of PGD(2) synthesis, while COX-1 (-/-) cells secrete low levels of PGD(2) between 2 and 4 h after activation. These data demonstrate that (i) the first phase of PG synthesis is COX-1 dependent and (ii) the second, delayed phase of PG synthesis is dependent on activation-induced synthesis and activity of COX-2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Cell Line
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Isoenzymes / deficiency
  • Isoenzymes / genetics*
  • Isoenzymes / metabolism*
  • Kinetics
  • Mast Cells / enzymology
  • Mast Cells / physiology*
  • Membrane Proteins
  • Mice
  • Mice, Knockout
  • Polymerase Chain Reaction
  • Prostaglandin D2 / biosynthesis
  • Prostaglandin-Endoperoxide Synthases / deficiency
  • Prostaglandin-Endoperoxide Synthases / genetics*
  • Prostaglandin-Endoperoxide Synthases / metabolism*
  • Prostaglandins / biosynthesis*
  • Time Factors


  • Isoenzymes
  • Membrane Proteins
  • Prostaglandins
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Ptgs1 protein, mouse
  • Prostaglandin D2