Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease

H Takahashi; T Iwata; Y Kitagawa; R H Takahashi; Y Sato; H Wakabayashi; M Takashima; H Kido; K Nagashima; K Kenney; C J Gibbs Jr; T Kurata

doi:10.1128/CDLI.6.6.983-985.1999

Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease

Clin Diagn Lab Immunol. 1999 Nov;6(6):983-5. doi: 10.1128/CDLI.6.6.983-985.1999.

Authors

H Takahashi¹, T Iwata, Y Kitagawa, R H Takahashi, Y Sato, H Wakabayashi, M Takashima, H Kido, K Nagashima, K Kenney, C J Gibbs Jr, T Kurata

Affiliation

¹ Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp

Abstract

We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

14-3-3 Proteins
Antibodies, Monoclonal
Antibody Specificity
Creutzfeldt-Jakob Syndrome / cerebrospinal fluid*
Creutzfeldt-Jakob Syndrome / diagnosis*
Humans
Hybridomas
Immunoblotting
Isomerism
Proteins* / chemistry
Proteins* / immunology
Proteins* / metabolism
Tyrosine 3-Monooxygenase*

Substances

14-3-3 Proteins
Antibodies, Monoclonal
Proteins
Tyrosine 3-Monooxygenase