A role for the deep orange and carnation eye color genes in lysosomal delivery in Drosophila

Mol Cell. 1999 Oct;4(4):479-86. doi: 10.1016/s1097-2765(00)80199-9.

Abstract

Deep orange and carnation are two of the classic eye color genes in Drosophila. Here, we demonstrate that Deep orange is part of a protein complex that localizes to endosomal compartments. A second component of this complex is Carnation, a homolog of Sec1p-like regulators of membrane fusion. Because complete loss of deep orange function is lethal, the role of this complex in intracellular trafficking was analyzed in deep orange mutant clones. Retinal cells devoid of deep orange function completely lacked pigmentation and exhibited exaggerated multivesicular structures. Furthermore, a defect in endocytic trafficking was visualized in developing photoreceptor cells. These results provide direct evidence that eye color mutations of the granule group also disrupt vesicular trafficking to lysosomes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carrier Proteins*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Endosomes / genetics
  • Eye Color / genetics*
  • Eye Proteins / genetics*
  • Fluorescent Antibody Technique
  • Fungal Proteins / chemistry
  • Insect Proteins / genetics
  • Lysosomes / genetics
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Mutation
  • Photoreceptor Cells, Invertebrate / ultrastructure
  • Saccharomyces cerevisiae Proteins*
  • Vesicular Transport Proteins*

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • DOR protein, Drosophila
  • Drosophila Proteins
  • Eye Proteins
  • Fungal Proteins
  • Insect Proteins
  • Saccharomyces cerevisiae Proteins
  • VPS33 protein, S cerevisiae
  • Vesicular Transport Proteins
  • car protein, Drosophila
  • hook protein, Drosophila

Associated data

  • GENBANK/AF133260