Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays

Mol Cell. 1999 Oct;4(4):649-55. doi: 10.1016/s1097-2765(00)80216-6.

Abstract

The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • CCAAT-Binding Factor*
  • Cell Cycle Proteins*
  • Chromatin / metabolism
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal / genetics*
  • Herpes Simplex Virus Protein Vmw65 / metabolism
  • Nuclear Proteins*
  • Nucleosomes / metabolism
  • Promoter Regions, Genetic*
  • Protein Kinases / metabolism
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation / genetics
  • Yeasts / metabolism

Substances

  • CCAAT-Binding Factor
  • Cell Cycle Proteins
  • Chromatin
  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • HAP4 protein, S cerevisiae
  • Herpes Simplex Virus Protein Vmw65
  • Nuclear Proteins
  • Nucleosomes
  • Recombinant Proteins
  • SWI5 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Protein Kinases