Differential Roles of TLR2 and TLR4 in Recognition of Gram-Negative and Gram-Positive Bacterial Cell Wall Components

Immunity. 1999 Oct;11(4):443-51. doi: 10.1016/s1074-7613(00)80119-3.

Abstract

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Bacterial / immunology*
  • Cell Wall / immunology*
  • Corynebacterium diphtheriae / chemistry
  • Corynebacterium diphtheriae / immunology
  • Drosophila Proteins*
  • Escherichia coli / chemistry
  • Escherichia coli / immunology
  • Female
  • Gram-Negative Bacteria / chemistry*
  • Gram-Negative Bacteria / immunology
  • Gram-Positive Bacteria / chemistry*
  • Gram-Positive Bacteria / immunology
  • Interleukin-1 Receptor-Associated Kinases
  • Lipid A / immunology
  • Lipopolysaccharides / immunology*
  • Macrophages, Peritoneal / immunology
  • Male
  • Membrane Glycoproteins / deficiency
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NF-kappa B / metabolism
  • Nocardia / chemistry
  • Nocardia / immunology
  • Peptidoglycan / immunology*
  • Protein Isoforms / deficiency
  • Protein Isoforms / genetics
  • Protein Isoforms / physiology*
  • Protein Kinases / metabolism
  • Receptors, Cell Surface / deficiency
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology*
  • Salmonella / chemistry
  • Salmonella / immunology
  • Signal Transduction
  • Species Specificity
  • Staphylococcus aureus / chemistry
  • Staphylococcus aureus / immunology
  • Teichoic Acids / immunology*
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors

Substances

  • Antigens, Bacterial
  • Drosophila Proteins
  • Lipid A
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • NF-kappa B
  • Peptidoglycan
  • Protein Isoforms
  • Receptors, Cell Surface
  • Teichoic Acids
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors
  • lipoteichoic acid
  • Protein Kinases
  • Interleukin-1 Receptor-Associated Kinases