C5a receptor expression by TGW neuroblastoma cells

Neuroreport. 1999 Sep 29;10(14):3021-5. doi: 10.1097/00001756-199909290-00027.

Abstract

We recently reported that not only lymphoid cells, but cells of neuronal origin may harbor C5a receptors (C5aR) as suggested by results of RT-PCR testing and that an apoptotic pathway is associated with the C5aR. To determine whether C5aR is expressed as an integral membrane protein, we generated mono- and polyclonal anti-C5aR antibodies. Flow cytometry showed a low-level expression of C5aR in TGW neuroblastoma cells. Epitope mapping suggested that a conformation change in C5aR occurs when exposed to C5a. Although an aphysiologically high concentration of C5a is necessary for inducing a transient increase in the intracellular Ca2+ level, TGW cells do employ the signal transduction pathway associated with C5aR, suggesting that these cells may serve as putative model for C5aR-expressing neurons.

MeSH terms

  • Antigens, CD / biosynthesis*
  • Brain Neoplasms / metabolism*
  • Calcium / metabolism
  • Complement C5a / metabolism*
  • Flow Cytometry
  • Humans
  • Immunoglobulin M / biosynthesis
  • Immunoglobulin M / genetics
  • In Situ Hybridization
  • Neuroblastoma / metabolism*
  • RNA, Messenger / biosynthesis
  • Receptor, Anaphylatoxin C5a
  • Receptors, Complement / biosynthesis*
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Antigens, CD
  • Immunoglobulin M
  • RNA, Messenger
  • Receptor, Anaphylatoxin C5a
  • Receptors, Complement
  • Tumor Necrosis Factor-alpha
  • Complement C5a
  • Calcium