Role of protein kinases A and C in the induction of mGluR-dependent long-term depression in the medial perforant path of the rat dentate gyrus in vitro

Neurosci Lett. 1999 Oct 22;274(2):71-4. doi: 10.1016/s0304-3940(99)00561-3.


The involvement of protein kinases A and C in the induction of low frequency stimulation-induced long-term depression (LTD) in the medial perforant path of the dentate gyrus in vitro has been studied using the selective PKA inhibitors H-89 and KT 5720 and PKC inhibitors Bisindolylmaleimide and Ro-31-8220. The PKC inhibitors Bisindolylmaleimide I and Ro-31-8220 and the PKA inhibitors H-89 and KT5720 all partially inhibited LTD induction. However, the presence of both a PKC and a PKA inhibitor was necessary to completely block LTD induction. The induction of long-term potentiation was not blocked by the inhibitors. It is suggested that the induction of LTD by LFS involves activation of PKC and PKA following activation of group I and group II metabotropic glutamate receptors (mGluR).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbazoles*
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Dentate Gyrus / chemistry
  • Dentate Gyrus / enzymology
  • Enzyme Inhibitors / pharmacology
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • In Vitro Techniques
  • Indoles / pharmacology
  • Isoquinolines / pharmacology
  • Long-Term Potentiation / physiology*
  • Maleimides / pharmacology
  • Neural Inhibition / physiology
  • Perforant Pathway / chemistry
  • Perforant Pathway / enzymology*
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism*
  • Pyrroles / pharmacology
  • Rats
  • Receptors, Metabotropic Glutamate / physiology*
  • Sulfonamides*


  • Carbazoles
  • Enzyme Inhibitors
  • Indoles
  • Isoquinolines
  • Maleimides
  • Pyrroles
  • Receptors, Metabotropic Glutamate
  • Sulfonamides
  • KT 5720
  • Cyclic AMP-Dependent Protein Kinases
  • Protein Kinase C
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • bisindolylmaleimide
  • Ro 31-8220