Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype

J Immunol Methods. 1999 Aug 31;228(1-2):13-21. doi: 10.1016/s0022-1759(99)00089-7.


Background and objectives: Freezing is a practical approach for cell preservation for retrospective studies. The aim of this work was to check the cryopreservation impact on B cell chronic lymphocytic leukaemia phenotype.

Material and methods: Blood samples from 15 CLL patients were analyzed freshly and after freezing at -196 degrees C, without separation, and thawing. Results were compared by Student's paired t-test.

Results: The phenotype of fresh CLL cells was as follows: CD19+, CD5+, faint CD20, CD23+/-, weak CD22 and sIg, CD37+, HLA-DR+, FMC7-. After cryopreservation, the percentage of CD5 and CD23 positive cells decreased, whereas HLA-DR positive cells increased moderately. The CLL Matutes's score was modified in 6 cases out of 15 (40%).

Conclusion: Cryopreservation modifies B cell chronic lymphocytic leukaemia phenotype, by decreasing CD5 and CD23 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / blood
  • B-Lymphocytes / immunology
  • Blood Preservation*
  • CD5 Antigens / blood
  • Cryopreservation*
  • Evaluation Studies as Topic
  • Flow Cytometry
  • HLA-DR Antigens / blood
  • Humans
  • Immunoglobulin kappa-Chains / blood
  • Immunoglobulin lambda-Chains / blood
  • Immunophenotyping
  • Leukemia, B-Cell / blood*
  • Leukemia, B-Cell / immunology*
  • Receptors, IgE / blood


  • Antigens, CD
  • CD5 Antigens
  • HLA-DR Antigens
  • Immunoglobulin kappa-Chains
  • Immunoglobulin lambda-Chains
  • Receptors, IgE