Deletogenic activity of 1,2:7,8-diepoxyoctane in the Salmonella typhimurium tester strain TA102

Mutat Res. 1999 Sep;437(2):165-73. doi: 10.1016/s1383-5742(99)00082-4.

Abstract

1,2:7,8-Diepoxyoctane (DEO), whose deletogenic activity was first demonstrated in ad-3 system of Neurospora crassa and then in different species, has been tested in Salmonella typhimurium tester strain TA102 (hisG428(Ochre)). It was confirmed that it is a direct acting mutagen and was found that its activity is stimulated with the S9 mix. Obtained His(+) revertants were screened on their response to the histidine analog, N-(2-thiazolyl)-DL-alanine (ThiAla). Thirty-two percent of spontaneous and 52% of DEO-induced revertants were resistant to the analog while no resistance was observed among those induced with 4-nitroquinoline-N-oxide (4NQO). Resistance to ThiAla was interpreted as due to small deletions surrounding the target TAA codon in hisG428(Ochre). Thus, at least two simple test-systems, ad-3 of N. crassa and hisG428(Ochre) of S. typhimurium, gave compatible results and might be useful in searching of deletogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylating Agents / toxicity
  • Animals
  • Base Sequence
  • DNA, Bacterial / drug effects
  • DNA, Bacterial / genetics
  • DNA, Bacterial / radiation effects
  • Epoxy Compounds / toxicity*
  • Genes, Bacterial / drug effects
  • Genes, Bacterial / radiation effects
  • In Vitro Techniques
  • Microsomes, Liver / metabolism
  • Mutagenicity Tests
  • Mutagens / toxicity*
  • Mutation
  • Rats
  • Salmonella typhimurium / drug effects*
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / radiation effects
  • Sequence Deletion

Substances

  • Alkylating Agents
  • DNA, Bacterial
  • Epoxy Compounds
  • Mutagens
  • 1,2,7,8-diepoxyoctane