Groalpha-mediated recruitment of neutrophils is required for elicitation of contact hypersensitivity

Eur J Immunol. 1999 Nov;29(11):3485-95. doi: 10.1002/(SICI)1521-4141(199911)29:11<3485::AID-IMMU3485>3.0.CO;2-B.


The factors mediating recruitment of immune T cells to challenge sites during contact hypersensitivity (CHS) responses remain unclear. To investigate the role of chemokines during elicitation of CHS, the temporal expression of chemokine genes in hapten-challenged ears was tested. KC (the murine homologoue of Groalpha) was expressed 30 min following hapten challenge in naive and hapten-sensitized mice. A rabbit KC-specific antiserum inhibited elicitation of CHS when administered to sensitized mice prior to hapten challenge. Injecting either neutrophils or immune CD8(+) T cells into the ear tissue of immune animals before hapten challenge circumvented the KC antiserum-mediated inhibition of CHS. Neutrophil depletion also inhibited CHS and was circumvented by injecting either neutrophils or hapten-primed CD8(+) T cells into ears of sensitized mice followed by specific hapten challenge. These results indicate that KC-directed neutrophil infiltration of hapten challenge sites is required for elicitation of CHS and suggest that neutrophils mediate recruitment of the hapten-specific CD8(+) T cells that subsequently produce cytokines mediating the hypersensitivity response.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chemotactic Factors / biosynthesis
  • Chemotactic Factors / immunology*
  • Dermatitis, Allergic Contact / immunology*
  • Growth Substances / biosynthesis
  • Growth Substances / immunology*
  • Haptens / immunology
  • Intercellular Signaling Peptides and Proteins*
  • Keratinocytes / immunology
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils / immunology*
  • Rabbits


  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl1 protein, mouse
  • Growth Substances
  • Haptens
  • Intercellular Signaling Peptides and Proteins