Abstract
Calcium plays a critical part in the regulation of cell growth, and growth factors stimulate calcium entry into cells through calcium-permeable channels. However, the molecular nature and regulation of calcium-permeable channels are still unclear at present. Here we report the molecular characterization of a calcium-permeable cation channel that is regulated by insulin-like growth factor-I (IGF-I). This channel, which we name growth-factor-regulated channel (GRC), belongs to the TRP-channel family and localizes mainly to intracellular pools under basal conditions. Upon stimulation of cells by IGF-I, GRC translocates to the plasma membrane. Thus, IGF-I augments calcium entry through GRC by regulating trafficking of the channel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Androstadienes / pharmacology
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Animals
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Calcium / metabolism*
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Calcium Channels / chemistry
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Calcium Channels / genetics
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Calcium Channels / physiology*
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Cattle
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Cell Membrane / physiology*
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Chromones / pharmacology
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Cloning, Molecular
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Cytosol / metabolism
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / physiology*
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Humans
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Insulin-Like Growth Factor I / pharmacology*
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Insulin-Like Growth Factor I / physiology
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Membrane Potentials / drug effects
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Mice
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Molecular Sequence Data
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Morpholines / pharmacology
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Phosphatidylinositol 3-Kinases / metabolism
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Phylogeny
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Rats
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Sequence Alignment
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Sequence Homology, Amino Acid
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TRPV Cation Channels
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Transfection
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Wortmannin
Substances
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Androstadienes
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Calcium Channels
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Chromones
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Morpholines
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Recombinant Fusion Proteins
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TRPV Cation Channels
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TRPV2 protein, human
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Trpv2 protein, mouse
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2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
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Insulin-Like Growth Factor I
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Phosphatidylinositol 3-Kinases
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Calcium
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Wortmannin